Q&A

QWhat is the minimum order in terms of number of sequences, and what is the smallest synthesis scale you offer?

AWe can accept orders starting from one sequence, and we offer synthesis at scales beginning in the nmol range. Please contact us via the inquiry form for details.

QWhat is the maximum synthesis scale you can support?

AAt SC-AMSA, we can support up to 2 mg per sequence. If you need larger quantities, we can coordinate within the Sumitomo Chemical Group located in Japan. Please contact us via the inquiry form for details.

QIs there a limit to the oligo length you can synthesize?

AWe have demonstrated synthesis capability up to 250mer. For longer sequences, we may be able to propose alternative approaches (e.g., ligation-based methods). Please contact us via the inquiry form for details.

QWhat types of chemical modifications do you support?

AWe support a wide range of commonly used chemical modifications, including 2′-O-Methyl (2′-OMe), 2′-Fluoro (2′-F), 2′-O-Methoxyethyl (2′-MOE), Locked Nucleic Acid (LNA), and phosphorothioate (PS) bonds. If you need other modifications, please contact us via the inquiry form.

QCan you support GMP manufacturing?

AGMP manufacturing is handled at Sumitomo Chemical’s Japan site (link). Our manufacturing method can be seamlessly scaled up to GMP manufacturing. Please contact us via the inquiry form for details.

QWhat is the typical lead time?

AFor in-vivo grade samples, typical lead time is around one month, depending on lab scheduling. Please contact us via the inquiry form for details.

QIn what form do you ship gRNA?

AOur standard shipping form is lyophilized (freeze-dried). If requested, we can also ship gRNA as a frozen aqueous solution. Please contact us via the inquiry form for details.

QHow do you handle confidentiality?

AWe handle your project details and sequence information under strict confidentiality, and we can execute an NDA when needed.

QCan you share the analytical results for the sample?

AYes. We provide an Analytical Data Report (ADR) with delivery. Please contact us via the inquiry form for analytical items.

QWhat is the difference between TBDMS amidite and PMM amidite?

APMM amidites typically provide higher coupling efficiency and are well-suited for longer RNA synthesis. In addition, TBDMS-based synthesis can generate more 2′–5′ linkage isomers whereas PMM-based synthesis can largely avoid these.

QDo you sell PMM amidites or solid supports?

ANo. We do not sell PMM amidites or solid supports.